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recombinant proteins affi gel blue gel beads bio rad  (Bio-Rad)


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    Bio-Rad recombinant proteins affi gel blue gel beads bio rad
    Recombinant Proteins Affi Gel Blue Gel Beads Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1510 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant proteins affi gel blue gel beads bio rad/product/Bio-Rad
    Average 96 stars, based on 1510 article reviews
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    96/100 stars

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    Wls-dependent canonical Wnt signaling regulates Notch signaling activity. A, TopGal staining for canonical Wnt activity in E12.5 and E14.5 tongues. B, in situ hybridization for Lef1 and Axin2 expression in E12.5 tongues. C, immunostaining of <t>Jag1</t> and active Notch1 in embryonic tongues. D, in situ hybridization for the expression of Notch3 and Hes1 in E12.5 and E14.5 tongues. Arrowheads and arrows indicate positive immunostaining or gene expression in epithelium and the underlying tissue, respectively. Scale bars, 250 μm (A and D); 400 μm (B); and 100 μm (insets for B and C).
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    Wls-dependent canonical Wnt signaling regulates Notch signaling activity. A, TopGal staining for canonical Wnt activity in E12.5 and E14.5 tongues. B, in situ hybridization for Lef1 and Axin2 expression in E12.5 tongues. C, immunostaining of Jag1 and active Notch1 in embryonic tongues. D, in situ hybridization for the expression of Notch3 and Hes1 in E12.5 and E14.5 tongues. Arrowheads and arrows indicate positive immunostaining or gene expression in epithelium and the underlying tissue, respectively. Scale bars, 250 μm (A and D); 400 μm (B); and 100 μm (insets for B and C).

    Journal: The Journal of Biological Chemistry

    Article Title: A Wnt/Notch/Pax7 signaling network supports tissue integrity in tongue development

    doi: 10.1074/jbc.M117.789438

    Figure Lengend Snippet: Wls-dependent canonical Wnt signaling regulates Notch signaling activity. A, TopGal staining for canonical Wnt activity in E12.5 and E14.5 tongues. B, in situ hybridization for Lef1 and Axin2 expression in E12.5 tongues. C, immunostaining of Jag1 and active Notch1 in embryonic tongues. D, in situ hybridization for the expression of Notch3 and Hes1 in E12.5 and E14.5 tongues. Arrowheads and arrows indicate positive immunostaining or gene expression in epithelium and the underlying tissue, respectively. Scale bars, 250 μm (A and D); 400 μm (B); and 100 μm (insets for B and C).

    Article Snippet: For the bead implantation experiment, explanted tongues at E14.5 were implanted with Affi-Gel blue agarose beads carrying Jag1 protein active peptide fragment (AnaSpec), harvested after 1 day in culture, and processed for paraffin sections for immunohistochemistry analysis.

    Techniques: Activity Assay, Staining, In Situ Hybridization, Expressing, Immunostaining, Gene Expression

    Reciprocal interaction of Wnt and Notch signaling regulates embryonic tongue development. A and B, immunostaining analysis for Pax7 (A) and pHH3 (B) expression in the tongue explants implanted with Jag1 or BSA-soaked beads. C and D, quantitation of the number of Pax7-positive (C) and pHH3-positive (D) cells in the tongue explants. E, quantitative analysis of the myogenic gene expression in tongue explants after incubation with or without DAPT. F, -fold enrichment of RBP-J-binding sites (S1 and S2) and control site (CS) of the Pax7 promoter region following ChIP with a Notch1 antibody. G, BATGAL staining for canonical Wnt activity in tongue explant cultured with or without DAPT. H, schematic showing a genetic hierarchy that regulates embryonic tongue development. Data are shown as scatter plots. **, p < 0.01. Scale bars, 100 μm (A and B) and 200 μm (G).

    Journal: The Journal of Biological Chemistry

    Article Title: A Wnt/Notch/Pax7 signaling network supports tissue integrity in tongue development

    doi: 10.1074/jbc.M117.789438

    Figure Lengend Snippet: Reciprocal interaction of Wnt and Notch signaling regulates embryonic tongue development. A and B, immunostaining analysis for Pax7 (A) and pHH3 (B) expression in the tongue explants implanted with Jag1 or BSA-soaked beads. C and D, quantitation of the number of Pax7-positive (C) and pHH3-positive (D) cells in the tongue explants. E, quantitative analysis of the myogenic gene expression in tongue explants after incubation with or without DAPT. F, -fold enrichment of RBP-J-binding sites (S1 and S2) and control site (CS) of the Pax7 promoter region following ChIP with a Notch1 antibody. G, BATGAL staining for canonical Wnt activity in tongue explant cultured with or without DAPT. H, schematic showing a genetic hierarchy that regulates embryonic tongue development. Data are shown as scatter plots. **, p < 0.01. Scale bars, 100 μm (A and B) and 200 μm (G).

    Article Snippet: For the bead implantation experiment, explanted tongues at E14.5 were implanted with Affi-Gel blue agarose beads carrying Jag1 protein active peptide fragment (AnaSpec), harvested after 1 day in culture, and processed for paraffin sections for immunohistochemistry analysis.

    Techniques: Immunostaining, Expressing, Quantitation Assay, Gene Expression, Incubation, Binding Assay, Control, Staining, Activity Assay, Cell Culture